高分辨率熔解曲线分析应用的研究进展

Abstract

Abstract: High resolution melting analysis (HRMA) was a new molecular diagnosis method for mutation scanning and genotyping, which combined special saturated fluorescent dyes, unlabled probe and Real-time PCR. High resolution melting analysis was superior to other nucleic acid research methods, including an inexpensive closed tube format that was a rapid, accurate, sensitive, special and high-throughput assay. In this paper, advancement on the application of high resolution melting in molecular diagnosis was summarized.

Key words: mutation detection; melting curve analysis; nucleic acid

CLC Number:

S816.79

LIU Zi-zeng, WU Zhou-liang, YAN Ping, WANG Yi-wen. Research Progress on the Application of High-resolution Melting Curve Analysis[J]. , 2013, 40(8): 105-111.

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Research Progress on the Application of High-resolution Melting Curve Analysis

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