ENO1过表达对籽鹅卵泡颗粒细胞凋亡基因Bcl-2表达的影响

Abstract

Abstract: The paper was to study the effects of ENO1 overexpression with adenovirus vector on Bcl-2 in the granulose cell of Zi goose. Adenovirus vector with ENO1 were transfected to Zi goose primary cultured granulose cells, and Bcl-2 mRNA of granulose cells were detected by the method of Real-time fluorescence quantitative PCR. The results showed that ENO1 overexpression made the Bcl-2 mRNA expression in the granulose cells significantly increase (P<0.01). ENO1 overexpression made the primary culture follicle granulose cells in vitro improve Bcl-2 mRNA expression, which suggested that ENO1 might inhibit the apoptpsis of Zi goose granulose cell.

Key words: Zi goose; ENO1 overexpression; Bcl-2 gene; granulosa cells

CLC Number:

S835

WANG Jiang-lu, JI Hong, ZHANG Wei-hong, HE Rong-he, GUO Jing-ru, GUO Li, YANG Huan-min. Effects of ENO1 Overexpression with Adenovirus Vector on Bcl-2 in the Granulose Cell of Zi Goose[J]. , 2013, 40(7): 10-13.

References

1 王丹,郭景茹,刘胜军,等. 籽鹅卵巢产蛋性能相关基因全长cDNA序列的克隆与分析[J]. 中国生物制品学杂志,2010,23(12): 1320~1324.
2 迟万好,曹明富,朱睦元. 细胞凋亡的基因调控研究进展[J]. 杭州师范学院学报(自然科学版),2001,18(5): 46~47.
3 陈秀萍,陈锋剑,姜勋平,等. Bcl-2基因RNA干扰对体外培养的鹅卵泡颗粒细胞凋亡、增殖和孕激素分泌的影响[J]. 畜牧兽医学报,2008,39(3): 296~301.
4 姜勋平,周东蕊,杨利国,等. 鸡Bcl-2基因的克隆及其对卵泡颗粒细胞凋亡的影响[J]. 中国兽医学报,2002,22(1): 87~89.
5 范凌云,谢庆军. 腺病毒载体的研究进展[J]. 中国生物制品学杂志,2008,21(2): 153~157.
6 施勤,刘继明,张学光. 细胞凋亡的线粒体调控与Bcl-2基因家族[J]. 上海免疫学杂志,2000, 20(1): 60~61.
7 Alden T D, Pittman D D, Beres E J, et al. Percutaneous spinal fusion using bone morphogenetic protein-2 gene therapy[J]. J Neurosurg,1999,90(Suppl): 109~114.
8 Calvoa M N,Bartronsa R,Castano E,et al. PFKFB3 gene silencing decreases glycolysis, induces cell-cycle delay and inhibits anchorage-independent growth in HeLa cells[J]. FEBS Letters,2006,580(13): 3308~3314.
9 Degterev A, Lugovsroy A, Cardone M, et al. Identification of small-molecule inhibitors of interaction between the BH3 domain and Bcl-xL[J]. Nat Cell Bid,2001,3(2): 173~182.
10 Gursoy E,Ergin K,Basaloglu H,et al. Expression and localization of Bcl-2 and Bax proteins in developing rat ovary[J]. Resvetsci,2008,84(1): 56~61.
11 Hajnoczky G, Csordas G, Das S, et al. Mitochondrial calcium signaling and cell death: approaches for assessing the role of mitochondrial Ca²⁺ up take in apoptosis[J]. Cell Calcium, 2006, 40(5~6): 553~560.
12 Nutt L K, Margolis S S, Jensen M, et al. Metabolic regulation of oocyte cell death through the CaMKⅡ-mediated phosphorylation of caspase-2[J]. Cell, 2005, 123: 89~103.
13 Pancholi V. Multifunctionalα-enolase:Its role in disease[J]. Cell Mol Life Sci, 2001, 58:902~920.
14 Subram anian A, Miller D M. Structural analysis of alpha-enolsae mapping the functional domains involved in down-regulation of the c-myc protooncogene[J]. J Biol Chen, 2000, 275(8): 5958~5965.
15 Sugiura K, Pendola F L, Eppig J J. Oocyte control of metabolic cooperativity between oocytes and companion granulose cells: Energy metabolism[J]. Dev Biol, 2005, 279(1): 20~30.
16 Sutton-McDowall M L, Gilchrist R B, Thompson J G. The pivotal role of glucose metabolism in deter-mining oocyte develolmental competence[J]. Reproduction, 2010, 139: 685~695.

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Effects of ENO1 Overexpression with Adenovirus Vector on Bcl-2 in the Granulose Cell of Zi Goose

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