Abstract: Primers and probes were designed based on inserting sequence IS1111 of Q fever Coxiella burnetii, TaqMan Real-time quantitative PCR assay was developed for indentification Q fever. The recombinant plasmid containing the target sequence was constructed to detect the sensitivity and prepare the standard curve. The method could detect 10 of the plasmid copy number. Related coefficient was 0.995 of the standard curve, the amplification efficiency was 103%. The results of specific detection of nucleic acid sample for M. tuberculosis, Brucella. spp, C. psittaci and bovine blood were negative. TaqMan fluorescent quantitative PCR method was developed in this study and had high sensitivity and specificity. It had a good application prospects in the detection and identification of the Q fever.

Key words: Q fever; Coxiella burnetii; fluorescent quantitative PCR; TaqMan probe method