猪β2m四聚体前体链重组表达载体的构建和表达

Abstract

Abstract: In order to construct the tetrameric leader chain of swine β2m, the light chain of swine β2m was mutated at site of 69 from phenylalanine (Phe) to cysteine (Cys) by using PCR site-directed mutagenesis technique. Then the mutant β2m gene was inserted into pET-28a and transformed into Escherichia coli BL21. After induction, the expression of target protein was detected by SDS-PAGE. The results demonstrated that the mutant β2m was about 297 bp, with coding for 98 amino acids. It was shown that the phenylalanine at site of 69 was mutated to cysteine. After induction, the mutant β2m was expressed successfully as a protein of 10.6 ku detected by SDS-PAGE. It was concluded that the recombinant expression plasmids of the mutant β2m was constructed and target protein was expressed successfully. This study would lay the foundation for construction of tetramer later.

Key words: Beta2 microglobulin; tetramer; site-directed mutagenesis; expression

CLC Number:

LIU Chang-hua, LIU Fa, ZHANG Jing, QIAO Zhen, GAO Feng-shan . Construction and Expression of the Recombinant Expressing Plasmids for the Tetrameric Leader Chain of Swine β2m[J]. , 2013, 40(4): 77-81.

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Construction and Expression of the Recombinant Expressing Plasmids for the Tetrameric Leader Chain of Swine β2m

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