猪巨细胞病毒(PCMV)四川株gB基因的克隆与序列分析

Abstract

Abstract: A pair of specific primers was designed according to the sequence of porcine cytomegalovirus (PCMV) gB gene(FJ870563.1) in GenBank. The gB gene was amplified from the final diagnosed PCMV samples by PCR,the amplified fragments was cloned into the pMD19-T vectors,and the positive recombinant T-PCMV-gB was sequenced. The results showed that the complete gene was consisted of 2580 bp.The results of homology analysis showed that gB gene shared 98.0% to 99.6% homology with reference gB sequence, and 42.9% to 43.6%,40.5% to 40.6% of amino acid homology with HHV-6 and HHV-7 respectively. The effects of isolates owing to these amino acid substitutions on biological characterization were unknown.

Key words: porcine cytomegalovirus; gB gene; cloning; sequence analysis

CLC Number:

HE Wan-ping, ZHAO Ling, LIU Yan, LIU Yan, WU Yu-mei, XU Kai. Cloning and Sequence Analysis of Porcine Cytomegalovirus (PCMV) gB Gene from Sichuan[J]. , 2013, 40(3): 80-83.

References

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Cloning and Sequence Analysis of Porcine Cytomegalovirus (PCMV) gB Gene from Sichuan

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