›› 2013, Vol. 40 ›› Issue (3): 7-10.

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Cloning and Prokaryotic Expression of ccpA gene of Streptococcus suis Type 2

RAN Hui-ling1,2, LANG Xu-long2,3, WANG Xing-long2,3, ZHANG Rui2, QIAN Jing1, CHEN Si2, WANG Xiu-ran1   

  1. 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
    2. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China;
    3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China
  • Received:2012-09-27 Online:2013-03-20 Published:2013-03-19

Abstract: To obtain recombinant ccpA of Streptococcus suis type 2 expressed in prokaryotic system and preliminaryly evaluate its antigenicity and specificity by Western blotting.ccpA gene was obtained by PCR and cloned into pET-28a vector,then transformed into Escherichia coli BL21 to induce,express and purify,the antigenicity of recombinant ccpA was identified by Western blotting. The double enzyme analysis and sequencing proved that recombinant plasmid pET-28a-ccpA was constructed correctly.The expressed recombinant protein,with a relative molecular mass of about 38000,was recognized by positive serum antibody.The ccpA of Streptococcus suis type 2 was successfully expressed in E.coli,which provided a material for development of serological diagnostic method for Streptococcus suis type 2.

Key words: Streptococcus suis type 2; ccpA gene; prokaryotic expression

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