动物性食品源大肠杆菌O血清型鉴定及其K88菌毛基因检测

Abstract

Abstract: 20 strains of E.coli from meat, chickens and mutton collected from product market and supermarket of Hebei Jidong areas were to be identified with serotype identification and K88 fimbriae gene of different serotypes was detected. O serotype of the strains of E.coli were identified with conventional methods and PCR. These isolates distributed in 7 serogroups (O38,O78,O88,O11,O107,O91,O9) and O38 and O78 were the dominant serotypes, the positive rates were both 25%(5/20). Detection rate of K88 fimbriae gene from food was 30%(6/20).The serotypes of O78 and O38 in Jidong areas from food were commom, and the detection rate of K88 fimbriae gene from food was 30%.

Key words: E.coli from animal food; O serotype; K88 gene

CLC Number:

S852.612

ZHANG Yan-ying, GAO Gui-sheng, SHI Qiu-mei, GAO Guang-ping, LIU Shen. O Serotype Identification and K88 Fimbriae Genes Analysis of E.coli Isolated from Animal Foods[J]. , 2013, 40(3): 185-188.

References

1 王想霞,张青风,杜俊甫,等.濮阳市部分食品中3种致病菌污染状况调查[J].中国食品卫生杂志,2005,17(1):54～56.
2 刘书亮,王红宁,陶勇,等.四川省规模化猪场仔猪黄白痢E.coli分离鉴定[J].中国预防兽医学报,2001,23(5):367～371.
3 刘秀梵.抗大肠埃希氏菌粘着素K88的特异单克隆抗体的产生及其特性鉴定[J].江苏农学院学报,1985(6):1～6.
4 邹玲,任慧英,温建新,等.胶东地区肉鸡致病性大肠杆菌的分离及血清型鉴定[J].黑龙江畜牧兽医,2012,5:121～122.
5 周建平.襄樊市肉食品大肠杆菌污染状况的检测[J].安徽农学通报,2008,14(13):35～36.
6 周萍.细菌感染性腹泻研究进展[J].中国预防医学杂志,2006,7(4):359～361.
7 郝彦龙,剡根强,王静梅,等.新疆北疆地区致犊牛腹泻大肠杆菌的分离鉴定及部分生物学特性[J].中国兽医学报,2012,32(6):848～851.
8 高崧,刘秀梵.抗大肠埃希氏菌黏附素单克隆抗体诊断试剂的研制及其现场初步应用[J].畜牧兽医学报,1993,24(3):248～253.
9 甄盼盼,汤电,任艳娜,等. 食品动物源产CTX-M-14大肠杆菌传播分子机制的演变[J]. 中国畜牧兽医, 2012, 39(9): 195～202.
10 谭诗文,艾玉萍,冉懋韬,等.贵州羔羊致病性大肠杆菌血清型和热稳定性肠毒素的研究[J]. 贵州畜牧兽医,2002,26(6):4～5.
11 Dean N Y,Strom E A,Carey T A.A monoclonal antibody identifies 2134P fimbriae as adhesions an enterotoxigenicf Escterichia coli isolated from postweaning pigs[J].Vet Microbiol,1993,37(12):101.
12 Ewers C,Janssen T,Wieler L H.Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from collseptivemia in poultry[J]. Vet Microbiol,2004,104(1～2):91～101.
13 Kassenborg H D,Hedberg C W,Hoekstra M,et al.Farm visits and undercooked hamburgers as major risk factors for sporadic Escherichia coli O157:H7 infection:Data from a case-control study in 5 FoodNet sites[J].Clin Infect Dis,2004,38 (S3):S271～S278.
14 Nagy B,Fekete P Z.Enterotoxigenic Escherichia coli in veterinary medicine[J].Int J Med Microbiol,2005,295(6～7):443～454.
15 Ojeniyi B,Ahrens P,Meyhing A.Detection of fimbrial and toxin genes in Escherichia coli and their prevalence in piglets with diarrhea[J].Zenlralbl Veterinarmed,1994,419(10):49～59.
16 Orskov I, Orskov F.Serology of Escherichia coli fimbriae[J].Prog Allergy,1983,33:80～105.
17 Ouwehand A C,Conway P L.Purification and characterization of a component producted by Lactobacillus fermentum that inhibits the adhesion of K88 expressinhg Escherichia coli to porcine ideal mucus[J].Journal of Applied Bacteriology,1996,80(3):311～318.
18 Reeves P R,Wang L.Genomic organization of LPS specificloci[J].Curr Top Microbiol Immunol,2002,264(1):109～135.
19 Stenulz R,Weintraub A,Wildmalm G.The structers of Escherichia coli O-polysaccharide antigens[J].FEMS Microbiol Rev,2006,30:382～403.
20 Vandemaele F,Assadzadeh A,Derijcke J,et al.Avian pathogenic Escherichia coli (APEC)[J].Tijdschr Diergeneeskd,2002,127(19):582～588.
21 Varma J K,Greene K D,Reller M E,et al.An outbreak of Escherichia coli O157 infection following exposure to acont aminated building[J].J AMA,2003,290:2709～2712.
22 Whitfield C.Biosynthesis of lipopoly saccharide O antigens[J].Trends in Microbiology,1995,3:178～185.

O Serotype Identification and K88 Fimbriae Genes Analysis of E.coli Isolated from Animal Foods

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments