腹腔注射白消安对小鼠生殖能力影响的研究

›› 2013, Vol. 40 ›› Issue (12): 142-145.

Study on Effect of Fertility after Mice Treated with Busulfan by Intra-peritoneal Injection

WANG Chen^1,2, ZHU Hua-bin², QIN Yu-sheng², LIU Ling², HAO Hai-sheng², ZHAO Xue-ming², QIN Tong², LIU Jian-hua³, LI Zong-qiang¹, WANG Dong²

1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;
2. The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Husbandry Bureau of Aohan County, Chifeng City, Inner Monggolia Municipality, Chifeng 024300, China

Received:2013-05-14 Online:2013-12-20 Published:2014-02-11

Abstract

Abstract: The effect of fertility after mice treated with busulfan was observed and evaluated in this study. 20 sexually matured male ICR mice with 6 to 7 weeks old were randomly divided into 4 groups. Mice were treated with busulfan in doses of 40,45,50 mg/kg by intra-peritoneal injection, respectively, and perform a blank determination with 50% DMSO. Busulfan-treated mice mated with female 15 times after injection in phases, and we recorded the data of 460 females used for mating, such as conception rate, the litter size and the gender of 922 offspring. Then we measured male fertility after the mice were treated with differential dose by statistical analyses. The short-term infertility result from intra-peritoneal injection of busulfan, which can be recovered by themselves. The recovery of mice fertility treated with low doses was quickly than that with higher doses. The sex ratio of the offspring did not change by the treatment, though it made an impact to fertility.

Key words: busulfan; intra-peritoneal injection; fertility

CLC Number:

S814.1

WANG Chen, ZHU Hua-bin, QIN Yu-sheng, LIU Ling, HAO Hai-sheng, ZHAO Xue-ming, QIN Tong, LIU Jian-hua, LI Zong-qiang, WANG Dong. Study on Effect of Fertility after Mice Treated with Busulfan by Intra-peritoneal Injection[J]. , 2013, 40(12): 142-145.

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Study on Effect of Fertility after Mice Treated with Busulfan by Intra-peritoneal Injection

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