抗柱状黄杆菌多克隆抗体的制备及其免疫学特性鉴定

Abstract

Abstract: To prepare the polyclonal antibody against Flavobacterium columnaris, New Zealand rabbits were immunized with particulate antigen. Antisera from the immunized rabbits were purified with caprylic/ammonium sulfate precipitation. Titers and cross reaction of the polyclonal antibody were determined by indirect ELISA. The results showed that the protein concentration of the polyclonal antibody was 29.28 mg/mL. The titer was over 1:6.4×10⁴. No cross-reaction was observed with other aquatic pathogenic bacteria such as Edwardsiella tarda, E.coli, Aeromonas hydrophila, Vibrio anguillarum, Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio harveyi. High specific polyclonal antibody against Flavobacterium columnaris was prepared, which might be used for rapid detection of Flavobacterium columnaris.

Key words: Flavobacterium columnaris; polyclonal antibody; immunological properties

CLC Number:

S852.65

LV Na, ZHAO Guo-kun, SUN Qiang, ZHANG Hong-xi, YIN Xiao-ping, ZHANG Mao-lan, ZHANG Jie. Preparation of the Polyclonal Antibody against Flavobacterium columnaris and Identification of its Immunological Properties[J]. , 2013, 40(10): 64-67.

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Preparation of the Polyclonal Antibody against Flavobacterium columnaris and Identification of its Immunological Properties

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