牛病毒性腹泻病毒E0基因的原核表达与活性检测

Abstract

Abstract: A fragment of about 650 bp was amplified by RT-PCR technique with specific primers based on BVDV genome sequence. Then the target fragment was directionally cloned into pET32a vector. After identifying with enzyme cut and sequencing, the recombinant plasmid was transformed into E. coli BL21(DE3). The recombinant protein E0 was expressed in inclusion body form in E. coli after induction with IPTG. After purification, the purified protein was analyzed by Western blotting, the results showed that the purified recombinant protein retained better antigenicity.

Key words: bovine viral diarrhea virus; E0 gene; prokaryotic expression; characterization

CLC Number:

YAN Wei-hang. Expression and Characterization of E0 Protein of Bovine Viral Diarrhea Virus[J]. , 2013, 40(1): 54-56.

References

1 朱礼倩, 周艳君, 于海, 等. 牛病毒性腹泻在中国的流行现状分析[J].中国动物传染病学报,2011,19(5):83~86.
2 何延华, 黄新, 薄新文, 等. 牛病毒性腹泻病毒结构蛋白基因的克隆及其B细胞抗原表位的预测[J].新疆农业科学,2012, 49(2):310~317.
3 张芳芳, 王光华, 郑福英, 等. 青海牦牛病毒性腹泻病毒的分离与鉴定[J].中国兽医科学, 2011,41(11):1101~1105.
4 宫晓炜, 郑福英, 蔺国珍, 等. 牛病毒性腹泻病毒侵染细胞机制的研究进展[J].畜牧兽医学报,2012,43(4):503~508.
5 郜玉钢, 杜锐, 王全凯, 等. 牛病毒性腹泻病毒梅花鹿分离株E0基因的克隆与表达[J]. 中国兽医学报, 2005,25(4):353~355.
6 Abu Elzein E, Alkhalyifa M. Studies on BVD involving establishment of sentinel calves and assessment of herd immunity in a large dairy farm in Saudi Arabia[J]. Trop Anim Health Prod, 2012,44(3):413~417.
7 Burks J M, Zwieb C, Müller F, et al. Comparative structural studies of bovine viral diarrhea virus IRES RNA[J]. Virus Res, 2011,160(1~2):136~142.
8 Presi P, Struchen R, Knight J T, et al. Bovine viral diarrhea (BVD) eradication in Switzerland-experiences of the first two years[J]. Prev Vet Med, 2011,99(2~4):112~121.
9 Saa L R, Perea A, García-Bocanegra I, et al. Seroprevalence and risk factors associated with bovine viral diarrhea virus (BVDV) infection in non-vaccinated dairy and dual purpose cattle herds in Ecuador[J]. Trop Anim Health Prod, 2012,44(3):645~649.
10 Singh K, Miller M M, Kohrt L J, et al. Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair[J]. J Vet Sci, 2011,12(3):295~297.
11 Valle P S, Skjerve E, Martin S W, et al. Ten years of bovine virus diarrhea virus (BVDV) control in Norway: A cost-benefit analysis[J]. Prev Vet Med, 2005, 72(1~2):189~207.

[1]	JIAO Linlin, CHENG Yuting, WU Qingguo, WU Shuang, WU Zhi, ZHU Shanyuan, QIAN Yingjuan. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Tembusu Virus Capsid Protein [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2395-2402.
[2]	LI Can, LI Chuchu, ZENG Wei, ZHANG Ning, JI Chunxiao, CHEN Tao. Recombinant Expression and Function Study of RegⅢγ Protein in Porcine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2414-2426.
[3]	SONG Yue, SU Shengjie, ZHANG Fan, BAI Fan, DAI Lingli, WANG Na, WANG Dawei, YANG Qianwen, ZHAO Shihua, ZHANG Yuemei. Prokaryotic Expression and Polyclonal Antibody Preparation of Mannheimia haemolytica Truncated Leukotoxin [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2479-2486.
[4]	ZHANG Fangyuan, LIN Yating, YANG Dawei, CHEN Hu, LI Guimei, SHAN Hu. Prokaryotic Expression of African Swine Fever Virus P30 Protein and Establishment of Indirect ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2012-2022.
[5]	HU Leyu, HU Xinyao, LI Ying, ZHAO Shengmiao, SHEN Fengzhen, WANG Changfa, LI Liangliang, WANG Tongtong, REN Huiying. Bioinformatics Analysis of HO-1 Gene and Preparation of Polyclonal Antibody in Dezhou Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1499-1510.
[6]	WANG Wenjie, RU Penghui, YU Chuan, DU Fuxi, CHEN Songbiao, SHANG Ke, ZHANG Chunjie, CHENG Xiangchao. Prokaryotic Expression of VP2 Protein of Luoyang Feline Panleukopenia Virus Isolate and Polyclonal Antibody Preparation [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1511-1521.
[7]	LIU Jiaxing, HAN Xueying, ZHANG Xinru, DENG Jiaxin, YAO Lunguang, LIU Yangkun. Preparation and Immunogenicity Analysis of Ferritin Nanoparticles Carrying Antigenic Epitopes of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1567-1574.
[8]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[9]	ZHANG Zhitao, LI Xianglong, KONG Lingli, LUO Yuxin, WANG Dongying. Prokaryotic Expression of Serine/Threonine Protein Phosphatase 2A in Fasciola gigantica and Expression Analysis at Different Stages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1107-1117.
[10]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[11]	GAN Lu, ZHENG Huizhen, NUO Mingdalai, LIU Yan, JIN Min, HE Wenwen, WEN Licui, LI Yongchang, GAILIKE·Bayinchahan. Propagation of Trypanosoma evansi Yili Strain and Cloning, Expression and Bioinformatics Analysis of Its PFR Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 469-478.
[12]	LYU Shuangfei, MA Xun, WANG Jing, KONG Cuilian, KOU Lijun, LIU Caixia, SHI Weidi, KANG Lichao, REN Huijie. Prokaryotic Expression and Immunoreactivity of PA-LF1 Fusion Protein of Bacillus anthracis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 674-683.
[13]	ZHANG Kai, ZHANG Chunping, GE Shengqiang, SUN Chunxi, CHENG Jie, FU Chunyu, WANG Gang, NIU Xing, PENG Jun. Expression of p22 Protein of African Swine Fever Virus,Preparation of Polyclonal Antibody and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 270-279.
[14]	ZHU Lilin, WANG Houkun, CHEN Xueyang, LIU Jing, LIANG Xiongyan, FANG Chun, YANG Yuying. Effect of Chicken IFIT5 on Replication of J Subgroup Avian Leukemia Virus Within DF-1 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 310-316.
[15]	ZHAO Fengmiao, GUO Ting, ZHOU Yaping, ZHAO Hongmei, WANG Yuchen, TIAN Guangyuan, SUN Yajie, BIAN Yuchen, YU Jialiang, HAO Yongqing. Pathogenicity Analysis of Bovine Viral Diarrhea Virus and Immune Effect Evaluation of E2 Protein in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3549-3558.

Expression and Characterization of E0 Protein of Bovine Viral Diarrhea Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments