›› 2013, Vol. 40 ›› Issue (1): 17-21.

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Effect of Herba Cistanchis on the Expression of the BMP2 Gene in Rat Osteoblasts

XING Xiao-xu1, LIU Zhong-jie2, HAN Bo2   

  1. 1. Taiyuan Zoo, Taiyuan 030009, China;
    2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
  • Received:2012-04-06 Online:2013-01-20 Published:2013-01-14

Abstract: The objection of the study was to investigate the effect of Herba Cistanchis aqueous extracts on the bone morphogenetic protein 2 (BMP2) gene expression in rat osteoblasts. Osteoblasts were isolated from calvaria in neonatal rat and exposure to different doses of Herba Cistanchis aqueous extracts(5?10-5 to 5?10-1 mg/mL).RNA was isolated from osteoblasts cultured and BMP2 gene expression was detected by Real-time fluorescent quantitative PCR. The results indicated that Herba Cistanchis aqueous extracts stimulated BMP2 gene expression.

Key words: Herba Cistanchis; osteoblast; BMP2 gene; fluorescent quantitative PCR

CLC Number: