兔病毒性出血症病毒重组蛋白VP60间接ELISA抗体检测方法的建立与应用

Abstract

Abstract: To develop a method to detect the antibody of rabbit hemorrhagic disease virus, an indirect ELISA was successfully developed to detect anti-RHDV antibody using recombinant VP60 protein as coating antigen. The method detected the positive sera of other three rabbit diseases(LaRV,SeV,Cl.welchii) were negative. Lowest antibody titer of positive control serum was 1:12800. Coefficient of variability percent (C.V%) of intro-batch and inter-batch duplicative tests were less than 4.9% and 6.9%, respectively. Therefore, the VP60-ELISA had good specificity, sensitivity and repeatability, which provided a technical means of rapid diagnosis for RHDV antibody detection and epidemiological investigation.

Key words: rabbit hemorrhagic disease virus; VP60 protein; indirect ELISA

CLC Number:

S852.4

LI Yun-qin, ZHAO Mei-ying, DIAO Fu-hua. Development and Application of an Indirect ELISA for Detecting Antibodies to Rabbit Hemorrhagic Disease Virus Using Recombinant Truncated VP60 Protein[J]. , 2013, (7): 69-72.

References

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Development and Application of an Indirect ELISA for Detecting Antibodies to Rabbit Hemorrhagic Disease Virus Using Recombinant Truncated VP60 Protein

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