RNA干扰抑制家畜病毒感染的研究进展

Abstract

Abstract: RNA-mediated interference (RNAi) is a conserved gene-silencing mechanism, where by the doublestranded RNA matching is used as a signal to trigger the sequence-specific degradation of homologous mRNA. RNAi has become a valuable tool for analysis of gene function through suppression of specific gene products. In addition, RNAi has shown great promise for use in antiviral strategies designed to suppress the expression of pathogenic genes. The present review elaborates the mechanism of RNA interference, the development and application about RNAi inhibiting the virus replication and infection in animals, and the issued obstacles.

Key words: RNA-mediated interference; viral infection; replication inhibition

CLC Number:

S852.7

HUANG Shuang, WANG Feng-xue, WEN Yong-jun. Advancement on the Inhibition of Viral Replication by RNA Interference in Animals[J]. , 2013, (5): 61-66.

References

1 王安涛,陈创夫,乔军,等.靶向山羊痘病毒DNA聚合酶基因shRNA质粒表达载体的构建及筛选[J].中国细胞生物学学报,2011,33:520~525.
2 王海燕,刘文博,高崧,等.载体表达的siRNA分子对猪圆环病毒2型复制的抑制作用[J].微生物学报,2008,48:1507~1513.
3 王凤雪,师新川,温永俊,等.高致病性PRRSV Nsp2基因RNA干扰对病毒复制的影响[J].生物技术通报, 2012(5):132~137.
4 刘惠莉,陆承平,朱伟云.鸡传染性支气管炎病毒的RNA干扰[J].中国病毒学,2005,20:272~276.
5 宋德武,李志杰,王晓莉,等.RNAi靶向GP5基因抑制猪繁殖与呼吸综合征病毒在MARC-145细胞中的复制[J].中国兽医学报,2010,30:1013~1017.
6 周俊芳,华修国,崔立,等.shRNA表达质粒体外防治猪传染性胃肠炎病毒感染[J].上海交通大学学报,2009,27:24~27.
7 杨闽楠,李志杰,丁壮,等.RNAi靶向N基因抑制猪繁殖与呼吸综合征病毒的复制[J].中国兽医学报,2010,30:1571~1579.
8 贺云霞,华荣虹,周艳君,等.猪繁殖与呼吸综合征病毒ORF2~4特异siRNA的筛选及其抑制病毒复制效果的研究[J].生物工程学报,2007,23:794~800.
9 夏银河,潘耀谦,朱广蕊,等.沉默病毒编码的miRNA——新型抗病毒的治疗法研究进展[J].中国畜牧兽医,2012,39(2):35~39.
10 黄娟,姜平,李玉峰,等.M蛋白基因shRNA抑制PRRSV在Marc145细胞中复制的研究[J].中国农业科学,2008,41:259~264.
11 康洁,李淑萍.RNAi对猪瘟病毒Ns5B基因表达的抑制作用[J].中国兽医杂志,2004,40:23~25.
12 Bao Y, Guo Y, Zhang L,et al.Inhibition of porcine reproductive and respiratory syndrome virus replication by RNA interference in MARC-145 cells. Molecular Biology Reports,2012, 39(3):2515~2522.
13 Brummelkamp T R, Bernards R, Agami R.Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell,2002a, 2(3):243~247.
14 Brummelkamp T R,Bernards R, Agami R.A system for stable expression of short interfering RNAs in mammalian cells. Science,2002b, 296(5567):550~553.
15 Chen F Y, Wang H M, Yang S H, et al.ShRNA against NSP4 gene inhibits the proliferation of bovine rotavirus in vitro. Chinese Journal of Virology,2010,26(3):244~248.
16 Chen M, Payne W S, Hunt H, et al.Inhibition of Marek’s disease virus replication by retroviral vector-based RNA interference. Virology,2008, 377(2):265~272.
17 Chen W, Yan W, Du Q, et al.RNA interference targeting VP1 inhibits foot-and-mouth disease virus replication in BHK-21 cells and suckling mice. J Virol,2004,78(13):6900~6907.
18 Daude N, Marella M, Chabry J.Specific inhibition of pathological prion protein accumulation by small interfering RNAs. J Cell Sci,2003,116(Pt13):2775~2779.
19 Elbashir S M, Harborth J, Lendeckel W, et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature,2001,411(6836):494~498.
20 Ferreira H L, Spilki F R, De Almeida R S, et al.Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells. Antiviral Research,2007, 74(1):77~81.
21 Gao Y, Liu W, Gao H, et al.Effective inhibition of infectious bursal disease virus replication in vitro by DNA vector-based RNA interference. Antiviral Research,2008, 79(2):87~94.
22 Ge Q, Filip L, Bai A, et al.Inhibition of influenza virus production in virus-infected mice by RNA interference.Proc Natl Acad Sci USA,2004, 101(23):8676~8681.
23 He Y X, Hua R H, Zhou Y J, et al.Interference of porcine reproductive and respiratory syndrome virus replication on MARC-145 cells using DNA-based short interfering RNAs. Antiviral Research,2007, 74(2):83~91.
24 Kim S M, Lee K N, Park J Y, et al.Therapeutic application of RNA interference against foot-and-mouth disease virus in vitro and in vivo. Antiviral Research,2008, 80(2):178~184.
25 Lambeth L S, Moore R J, Muralitharan M S, et al.Suppression of bovine viral diarrhea virus replication by small interfering RNA and short hairpin RNA-mediated RNA interference. Veterinary Microbiology,2007, 119(2~4):132~143.
26 Li J N, Guo H C, Shi Z X, et al.In vitro inhibition of CSFV replication by retroviral vector-mediated RNA interference. Journal of Virological Methods,2010, 169(2):316~321.
27 Luo J H, Du J Z, Gao S D, et al.Lentviral-mediated RNAi to inhibit target gene expression of the porcine integrin alpha v subunit, the FMDV receptor, and against FMDV infection in PK-15 cells. Virology Journal,2011,8:428.
28 Lv K, Guo Y, Zhang Y, et al.Transient inhibition of foot-and-mouth disease virus replication by siRNAs silencing VP1 protein coding region. Research in Veterinary Science,2009, 86(3):443~452.
29 Mallanna S K, Rasool T J, Sahay B, et al.Inhibition of anatid herpes virus-1 replication by small interfering RNAs in cell culture system. Virus Research,2006, 115(2):192~197.
30 McCaffrey A P, Nakai H, Pandey K, et al.Inhibition of hepatitis B virus in mice by RNA interference. Nat Biotechnol,2003, 21(6):639~644.
31 Miyagishi M, Taira K.U6 promoter-driven siRNAs with four uridine 3'overhangs efficiently suppress targeted gene expression in mammalian cells. Nat Biotechnol,2002, 20(5):497~500.
32 Paddison P J, Caudy A A,Sachidanandam R, et al.Short hairpin activated gene silencing in mammalian cells. Methods Mol Biol,2004, 265:85~100.
33 Paul C P, Good P D, Winer I, et al.Effective expression of small interfering RNA in human cells. Nat Biotechnol,2002, 20(5):505~508.
34 Sohail M, Doran G, Riedemann J, et al.A simple and cost-effective method for producing small interfering RNAs with high efficacy. Nucleic Acids Res,2003,31(7):38.
35 Tompkins S M, Lo C Y, Tumpey T M, et al.Protection against lethal influenza virus challenge by RNA interference in vivo. Proceedings of the National Academy of Sciences of the United States of America,2004, 101(23):8682~8686.
36 Wang Y J, Sun H, Shen P, et al.Effective inhibition of replication of infectious bursal disease virus by miRNAs delivered by vectors and targeting the VP2 gene. Journal of Virological Methods,2010, 165:127~132.
37 White M D, Farmer M, Mirabile I, et al.Single treatment with RNAi against prion protein rescues early neuronal dysfunction and prolongs survival in mice with prion disease. Proceedings of the National Academy of Sciences of the United States of America,2008, 105(29):10238~10243.
38 Yin R F, Ding Z A, Liu X X, et al.Inhibition of Newcastle disease virus replication by RNA interference targeting the matrix protein gene in chicken embryo fibroblasts. Journal of Virological Methods,2010, 167(1):107~111.
39 Zhao L J, Jian H, Zhu H.Specific gene inhibition by adenovirus-mediated expression of small interfering RNA. Gene,2003, 316:137~141.
40 Zhao Z, Wu G, Zhu X, et al.RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells. Virology Journal,2012,9:48.
41 Zhou Y, Chan J H, Chan A Y, et al.Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells. FEBS Letters,2004, 577(3):345~350.

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Advancement on the Inhibition of Viral Replication by RNA Interference in Animals

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