Abstract: A diseases occurred in a beef cattle fattening farm in Urumqi area,characterised by higher body temperature,dyspnea,coughing and could cause rapid death,daily mortality more than 5%. One bacterial strain was isolated from liver and lung samples of new dead cattle,the bacterial was identified as Pasteurella hemolytica according to cultivation characters,colonial morphology,stain peculiarity and biochemistry test.5 experimental rats received intraperitoneal injection of the bacterial suspension,the result was that all of them died within 8 hours.Combine the epidemiological fact that these cattles just came to Urumqi after long-distance transportation from Yili,so the diagnosis of the disease was bovine shipping fever caused by Pasteurella hemolytica.Drug sensitivity test showed that sarafloxacin hydrochloride and doxycycline were most sensitive, we used these kinds of drugs to the whole herd,it showed good treatment and prevention effect.

Key words: bovine; Pasturella haemolytica; isolation; identificationORF5a