固醇调节元件结合蛋白基因的过表达对促进牛胎儿成纤维细胞脂肪形成的影响

Abstract

Abstract: The objective of this study was to investigate the relationship between sterol regulatory element binding factor 1 (SREBP1) gene expression and cell fat formation, recombinant plasmid pEGFP-C1-SREBP1 was constructed and transferred into bovine fetal fibroblasts with Lipofectamine 2000, and then the cells were cultured for 48 h. Furthermore, the expressions of SREBP1 mRNA and protein were detected by Real-time PCR and Western blotting. Oil red O staining was used to explore cellular lipid droplet formation. The results showed that an up-regulation of SREBP1 mRNA in recombinant plasmid transfection group compared with normal control group and pEGFP-C1 transfected group, SREBP1 protein also appeared obvious rise. Oil red O found no lipid droplet in normal control group and pEGFP-C1 transfected group, but marked lipid droplets were detected in pEGFP-C1-SREBP1 transfected group. In conclusion, the up-regulation of SREBP1 directly resulteds in cellular lipid droplet formation in bovine fetal fibroblasts.

Key words: sterol regulatory element binding factor 1; overexpression; bovine fetal fibroblasts; lipid droplet formation; plasmid transfection

CLC Number:

S816.79

XU Li, GUO Li-ping, ZHU Miao, GAO Xue, ZHANG Lu-pei, GAO Hui-jiang, LI Jun-ya, XU Shang-zhong. Effect of the Overexpression on Sterol Regulatory Element Binding Factor 1 Gene Increases Lipid Droplet Formation in Bovine Fetal Fibroblasts[J]. , 2013, (5): 133-139.

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Effect of the Overexpression on Sterol Regulatory Element Binding Factor 1 Gene Increases Lipid Droplet Formation in Bovine Fetal Fibroblasts

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