亚抑菌浓度穿心莲提取物对减缓金黄色葡萄球菌α-溶血素介导的A549细胞损伤作用的影响

Abstract

Abstract: Staphylococcus aureus was a widely spread opportunistic pathogen with α-hemolysin acting as the vital virulence factor, which could cause serious infection in both human beings and animals. Alcohol reflux was used to obtain the Chinese medicine common Andrographis herb extract. To analyze the effects of the medicine on the α-hemolysin secretion and A549 cell protection, assays including MIC determination, hemolysin assay, Western blotting, RT-PCR, LDH and Live/dead were undertaken. The results showed that the extract could inhibit the hemolysin secretion dose-dependently below the MIC of 2048 μg/mL, and effectively relieve the A549 damage by Staphylococcus aureus supernate, thus indicating it as a potential anti-Staphylococcus aureus drug.

Key words: Staphylococcus aureus; α-hemolysin; Andrographis paniculata

CLC Number:

S859.7

HUO Ming-fei, WANG Xin, DAI Xiao-han, DENG Xu-ming. Effects of the Subinhibitory Concentrations of Andrographis paniculata Extract on Inhibiting Staphylococcus aureus alpha-hemolysin Mediated A549 Cell Injury[J]. , 2013, (5): 110-114.

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Effects of the Subinhibitory Concentrations of Andrographis paniculata Extract on Inhibiting Staphylococcus aureus alpha-hemolysin Mediated A549 Cell Injury

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