产气荚膜梭菌ε毒素蛋白高效表达体系的构建及其免疫保护性的研究

Abstract

Abstract: The epsilon toxin produced by type B and D Clostridium perfringens, could lead to fatal illness in a variety of livestock animals, causing financial loss. In this study, pET-28a(+) plasmid harboring epsilon toxin gene was transferred into E.coli strain BL21(DE3)to efficiently produce epsilon toxin.Results of SDS-PAGE and Western blotting showed that recombinant E.coli BL21(DE3) could highly expressed ε toxin (about 32.83% of total cellular protein). On this basis, rabbits were immunized with 0.2 mg of the insoluble recombinant protein to evaluate immune protection of the expressed epsilon protein. Results of indirect ELISA demonstrated that anti-ε antibody titer reached the maximum value, 8.7log2 in the fourth week. In conclusion, the recombinant strain BL21(DE3) could highly express ε toxin, and the toxin had excellent immune protection.

Key words: Clostridium perfringens; epsilon toxin; expression; immune protection

CLC Number:

S852.61

LU Tian-fen, CHAI Tong-jie, SUN Ling-yu, LI Xin-xian, HAO Hai-yu, LV Chang-hui, YANG Yan-wei. Construction of Efficient Expression System of Clostridium perfringens Epsilon Toxin Protein and Analysis of its Immune Protection[J]. .

References

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Construction of Efficient Expression System of Clostridium perfringens Epsilon Toxin Protein and Analysis of its Immune Protection

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