猪IL-15多克隆抗体的制备及其生物活性的检测

Abstract

Abstract: Porcine IL-15 has a high similarity to human IL-15. Porcine IL-15 without signal peptide was cloned into prokaryotic expression vector pET-30a,the recombinant plasmid was transformed into E.coli BL21,and induced by IPTG. Recombinant protein was aquired successfully,then the purified protein was used to prepare polyclone antibody. Agarose diffusion assay,ELISA,Western blotting assy and immunofluorescence were used to detect the titer,specificity and sensitvity of the antibody. The results revealed that the polyclone antibody prepared in this study had a high titer and good specificity,and it could be used as the specific antibody to porcine IL-15 for the future research.

Key words: porcine IL-15; polyclonal antibody; biological activity

CLC Number:

Q511

ZOU Yu-fei, WANG Heng, YUAN Li-guo, LI Shou-jun. Preparation and Biological Activity Analysis of the Polyclone Antibody to Porcine IL-15[J]. .

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Preparation and Biological Activity Analysis of the Polyclone Antibody to Porcine IL-15

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