Abstract: This study was conducted to investigate the effect of different oligosaccharides and small peptides on the small intestinal villi histology of mice fed with high-fat diet. Seventy-two male ICR mice with initial average body weight of 24 g±2 g were randomly divided into 6 groups, which were fed with basal diet, high-fat diet, high-fat diet with 0.5% CP, 0.5% RP, 0.5% MOS and 0.5% COS, respectively. After 6 weeks, mice were killed to observe the mice’s small intestines in each group which were HE stained by light microscopy. The mice fed with high-fat diet lost small intestinal villus seriously and had a lower ratio of villus height and crypt depth than the control. All the high-fat diets with 0.5% CP, 0.5% RP, 0.5% MOS and 0.5% COS could ease the phenomenon of small intestinal villus’s shedding and short fusion, which not only increased the height of small intestinal villus, the ratio of villus height and crypt depth, but also shallowed small intestinal crypt depth compared with the high-fat diet groups. The protective effect of high-fat diet groups with 0.5% CP and 0.5% MOS on the protection of intestinal mucosa were the best.

Key words: high-fat; collagen peptides; rice peptides; mannose oligosaccharide; chitooligosaccharides; intestinal villi