›› 2012, Vol. 39 ›› Issue (7): 134-137.

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Study on Preparation and Immunocompetence of Inhibin Alpha-subunit in Beijing Duck

TIAN Jin   

  1. Beijing Vocational College of Agriculture,Beijing 102442,China
  • Received:2011-12-14 Online:2012-07-20 Published:2012-07-16

Abstract: pMD18-T vector with the inhibin α-subunit gene from Beijing duck and pET-28a expression vector were cut by NdeⅠ and XhoⅠ. The products were connected and transformated into E. coli BL21(DE3). The positive recombinant expression vectors were identificated by PCR and double digestion experiment. The inhibin α-subunit protein was induced by the IPTG. Tests in mice showed that inhibin α-subunit expressed effectively in E.coli BL21 had immunological competence. The results are helpful for the later research about improving the laying performance of Beijing duck.

Key words: Beijing duck; inhibin α-subunit gene; immunocompetence

CLC Number: