Abstract: To study the method of primary culture of cadiocytes in vitro using tissue explants of cardiac muscles isolated from neonatal Wistar rats. The hearts of the neonatal rats were removed and cut into small pieces, using the method of trypsinization isolated cell and cultivated in DMEM/F12 culture medium with 20% culture medium, daily observation under inverted phase contrast microscope. The results observed that several cells had the characteristic of rhythmic contraction after cultured 48 h; after 72 h, the cells gradually spread and the rhythmic contraction was 60 to 70 times/min and they were confirmed to be cadiocytes. Furthermore the cadiocytes were positive, which were identified by immunofluorescence with cTnT antibody and immunohistochemical strain of α-actin monoclonal antibody. This method of tissue culture is simple by which more single cadiocytes with good functional status can be obtained.

Key words: cadiocyte; separation culture; neonatal rat