›› 2012, Vol. 39 ›› Issue (4): 35-38.

• 生物技术 • Previous Articles     Next Articles

Expression of Fusion Gene Composed of Cecropin AD and Frog Buforin Ⅱ Mature Fragment in E.coli

HU Jin-dong1,2, WEI Yan-li1,2, ZHANG Guang-zhi1,2, LI Ji-shun1,2, YANG He-tong1,2,3   

  1. 1. Biotechnology Center,Shandong Academy of Sciences,Jinan 250014,China;2. Shandong Provincial Key Laboratory for Applied Microorganism,Jinan 250014,China;3. School of Life Sciences,Shandong University of Technology,Zibo 255049,China
  • Received:2011-10-10 Revised:1900-01-01 Online:2012-04-20 Published:2012-04-20

Abstract: The fragment composed of a cecropin AD and Buforin frog Ⅱ mature fragment was synthesized by using a modified two step total gene synthesis,then the fragment was cloned into pMD18-T and sequenced.Through restriction endonucleases digestion and were combinated into express vector pET-Trx,after transformed into E.coli BL21(DE3). The fusion gene was successly expressed. The quantity of expressed fusion protein accounted for probably 35% of total bacterial proteins and the fusion protein by the hydroxylamine cleavage exhibit obvious activities against the tested bacteria,for its further large-scale expression, purification and function to lay the foundation.

Key words: gene synthesis; cecropin AD; Buforin Ⅱ; construction of expression vector

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