猪Calsarcin-2基因第1内含子遗传多态性分析

Abstract

Abstract: In this study, the crossbreed and backcross of Yorkshire, Landrace, Duroc and Guizhou Bai Xiang pig were used to investigate the effect of Calsarcin-2 gene on meat quality. Four pairs of primers for intron 1 of Calsarcin-2 gene were designed according to pig sequences from GenBank. Polymorphisms were detected by PCR-SSCP method. SNPs were detected by DNA sequencing.2 SNPs(T613C,C936G) were identified. Population genetics analysis showed that the distribution of genotypes among five species were extremely significant difference (P＜0.01). Association of polymorphisms with breed characteristics indicated that the mutations of 613 and 936 sites may be related to meat quality.

Key words: pig; Calsarcin-2 gene; meat quality; PCR-SSCP

CLC Number:

S828.2

WU Xuan;XU Hou-qiang;NIE Yu-li;CHEN Xiang;ZHANG Yong. Genetic Polymorphism Analysis on Intron 1 of Calsarcin-2 in Porcine[J]. , 2012, 39(4): 139-142.

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Genetic Polymorphism Analysis on Intron 1 of Calsarcin-2 in Porcine

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