Abstract: To propagate SHIV-KB9 virus stock via passaged in Chinese-original monkey PBMCs and realize viral replication and immunological trauma in the monkey after inoculation intravenously with 10 fold dilution of SHIV-KB9. Infectious virus was generated by ligating pSHIV-KB9 5' and pSHIV-KB9 3', and transfecting CEMx174 cells. The peripheral blood mononuclear cells(PMBCs) from Chinese-original rhesus macaque were cocultured with the supernatant of transfection and the virus stock was collected when the viral replication reached the peak area.The variation of env gene was analized, so did the viral loads and tissue culture infective dose (TCID₅₀)with TZM-bl cells. Three Chinese-origin rhesus monkeys were infected with this batch of SHIV-KB9 intravenously with 10 fold dilution and the viral loads in plasma and the change of CD4⁺ and CD8⁺ T lymphocytes were analysed during the whole infection. In this study, totally 95 mL virus stock was propagated in monkeys PBMCs and without any variation about the gp120 sequence of env gene. The viral loads was 2.678×10⁵ copies/mL and had 3.16×10³ TCID₅₀ in 1 mL of cell-free SHIV-KB9 stock. 3 rhesus macaques were infected and presented severe and rapid depletion of CD4⁺ cells during acute phase. This panel of SHIV-KB9 adapted in Chinese-original rhesus monkeys' PBMCs were highly infectious and suitable for the animal modle.

Key words: SHIV-KB9; CCR5-Specifc; Chinese-original rhesus monkeys; PBMCs; TCID₅₀