›› 2012, Vol. 39 ›› Issue (2): 60-63.

• 生物技术 • Previous Articles     Next Articles

Detection of the Integration of Human Transferrin Gene on Chromosomes in Transgenic Cloned Cows

LIU Yu, ZHU Yi-wen, XU Miao, HUANG Ying   

  1. Key Laboratory of Medical Embryo and Molecular Biology of the Ministry of Health,Shanghai Key Laboratory of Embryo and Reproduction Engineering,Shanghai Institute of Medical Genetics,Children's Hospital of Shanghai,Shanghai 200040,China
  • Received:2011-06-22 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

Abstract: To detect the integration of human transferrin gene(hTF) on chromosomes in transgenic cloned cows,the author optimized fluorescence in situ hybridization(FISH). For transgenic cloned cows,53.1% to 86.8% of metaphases showed hybridization signals which were located on different chromosomes,such as Chr.11, Chr.15 and Chr.19. The results showed that using FISH to detect the integration site and hybridization signals was highly efficient and specific,also the copy numbers were detected by Real-time PCR.

Key words: fluorescence in situ hybridization; human transferring transgenic cloned cows; integration site; copy number

CLC Number: