China Animal Husbandry & Veterinary Medicine ›› 2012, Vol. 39 ›› Issue (2): 40-42.

• 生物技术 • Previous Articles     Next Articles

Real-time Quantitative RT-PCR Assay for Specific Identification of Panthera tigris Materials

ZHANG Tai-xiang1, LING Zong-shuai2, ZHAO Li-na1, YUAN Tao3, XU Biao3, LIANG Cheng-zhu3, LIU Wen-peng1, SUN Jun1, ZHANG Yi-bing1   

  1. 1. Weifang Entry-Exit Inspection and Quarantine Bureau of People's Republic of China, Weifang 261041, China;2. Jinan Entry-Exit Inspection and Quarantine Bureau of People's Republic of China, Jinan 250014, China;3. Shandong Entry-Exit Inspection and Quarantine Bureau of People's Republic of China,Qingdao 250023, China
  • Received:1900-01-01 Revised:2011-08-01 Online:2012-02-20 Published:2012-02-20

Abstract: Primers and probes were designed based on the conservative sequence of cytochondriome cytochrome b, a real-time quan quantitative RT-PCR assay was developed for indentification of Panthera tigris. The recombinant plasmid containing the target sequence was constructed to detect the sensitivity and prepare the standard curve. The real-time RT-PCR assay had a detection limit of 50 copies/μL, and with a dynamic range of detection between 5×105 to 5×101 copies /μL. The primers and probe were specific for Panthera tigris' DNA and did not react with other non- Panthera tigris' DNA. This real-time RT-PCR assay with high sensitivity, specificity and accuracy is considered to be a powerful tool for the rapid indentification and quantification of Panthera tigris.

Key words: Panthera tigris; real-time quantitative RT-PCR; TaqMan probe

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