Abstract: A strain of subgroup J avian leukosis virus(ALV-J) from Mahuang boiler chickens and another strain of ALV-J from Roman layer farms were isolated and identified by serologic test and PCR in Anhui province. The gp85 sequences deduced amino acid of two isolates had 82.1% to 99.4% homology to reference strains from subgroups J. The homology of the two isolates was 85.4%. Strain isolated from the Mahuang boiler chicken had 97.1% homology to strain HPRS-103 from British and 99.4% homology to strain SD09TA04 and SDYC02J ALV-J from China. The Roman layer flock isolated strain had 89.0% homology to HPRS-103 strain and 88.6% homology to strain SD09TA04 and SDYC02J ALV-J. The gp85 deduced amino acid sequences of two isolatess with variation and deletion, hypervariable regions of hr1、hr2 variants were significant. Evolutionary analysis further indicated that two isolates had distant relationship. The results showed that two isolates might come from different subgroups J avian leukosis virus.

Key words: subgroup J avian leukosis virus; strains isolated in Anhui provice; gp85 gene; sequence analysis