Abstract: In the experiments, collected the skin tissues of Xinjiang fine-wool sheep, extracted RNA, used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the KAP6.1 gene, and the recombination of the gene recombined into the pEGFP-C2 eukaryotic expression vector through the recombinant DNA technique, constructed the pEGFP-C2-KAP6.1 recombinant plasmid, then did the identification of PCR and enzyme digestion. The results showed that we successfully constructed the pEGFP-C2-KAP6.1 eukaryotic expression recombinant plasmid of Xinjiang fine-wool sheep. In order to further study the relationship of the protein encoding by the gene and the wool qualities, and provide a theoretical basis for the cultivation of economy biological characteristics interrelationship of superfine wool.

Key words: Xinjiang fine-wool sheep; pEGFP-C2 vector; KAP6.1 gene; eukaryotic expression