牛副流感病毒3型RT-LAMP检测方法的建立及应用

Abstract

Abstract: A set of primers used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) detection was designed based on the conserved nucleoprotein gene of bovine parainfluenza virus 3(BPIV-3) complete genome sequence submitted in GenBank. The usefulness of RT-LAMP for rapid preclinical detection of BPIV-3 infection was evaluated. The reaction could be finished in 1 h under isothermal condition at 63 ℃. This RT-LAMP assay had a detection limit of 0.069 fg/μL per reaction, was higher sensitivities than that of RT-PCR. The specificity of this assay could be easily confirmed by agarose gel electrophoresis, color reaction or turbidity comparison. As a result, the RT-LAMP assay was an ideal method for detecting BPIV-3 in laboratory and primary diagnosis of clinical infection.

Key words: bovine parainfluenza virus type 3; nucleoprotein gene; reverse transcription loop-mediated isothermal amplification

CLC Number:

S854.4

SHI Xin-chuan, WEN Yong-jun, WANG Feng-xue, HU Jia-xin, YANG Bo-chao, WANG Wei, SONG Ni, CHENG Shi-peng, WU Hua. Establishment and Application of Loop-mediated Isothermal Amplification for Bovine Parainfluenza Virus 3[J]. , 2012, 39(11): 31-34.

References

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Establishment and Application of Loop-mediated Isothermal Amplification for Bovine Parainfluenza Virus 3

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