几株鸡新城疫病毒的分离鉴定及其基因型分析

Abstract

Abstract: Six strains of virus isolated from several areas of poultry occurred Newcastle disease, through HA,HI,neutralization test, and molecular biology experiments were identified as Newcastle disease virus. According to F gene sequences published in GenBank, a pair of specific primers was designed and synthesized, length of approximately 610 bp was amplified, alignment and analysis of nucleotide and amino acid sequences were conducted. The results showed that there were 3 lines with virulent Newcastle disease virus strain characteristics, also had the Newcastle Ⅶ genotype features. According to MDT, ICPI and IVPI index determination result, we finally determined that the 3 strains were virulent strains of NDV and belonged to Ⅶ genotype. The homology of nucleic acid and amino acid sequence between remaining 3 strains of virus and La Sota were both 99%.

Key words: ND; F gene; virulent strain; genotype Ⅶ

CLC Number:

SUN Feng-ting, ZHENG Jie, ZHANG Fa-ming, CUI Hui-juan, YANG Guo-liang, ZHANG Hong, ZHANG Tan, ZHANG Liang, CHEN Ling, LI Jing, WANG Wen-quan. Isolation,Identification and Genotypic Analysis of Several Strains of Newcastle Disease Virus[J]. , 2012, 39(10): 76-79.

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Isolation,Identification and Genotypic Analysis of Several Strains of Newcastle Disease Virus

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