Abstract: The gB and gE gene of bovine herpesvirus-1 Bartha Nu/67 strain were amplified by PCR.The gB and gE fragment were cloned into pGEM-T-easy vector. The gB and gE gene were subcloned into baculovirus transfer vector and the recombinant Baculovirus vector pFBHgB, pFBHgE were constructed successfully. Then they were transferred into E.coli DH10Bac and cultured in LB plate. The white bacterial colonies were positive recombinant bacmid named as BacmidgB, BacmidgE. Thus the result mentioned above laid down theoretical and practical foundations for the expression of gB and gE gene in insect cells.

Key words: bovine herpesvirus-1(BHV-1); gB, gE gene; baculovirus expression vector system; vector construction