Abstract: The experiment was conduced to grope the electrical field strength and pulse width of porcine oocyte parthenogenetic and the effect of stage-culture method of osmotic pressure on parthenogentic embryo development. Afer 42 to 44 h of in vitro maturation, porcine oocytes were activated under the condition of 2.1, 2.3, 2.5 kV/mm and 30, 60, 90 μs, respectively. With using the parameter of 2.1 kV/mm and 30 μs for parthenogenetic activation, the oocytes were cultured in PZM-3 with the osmotic pressure of 271, 280, 290, 302 mOsm separately. Afer electrical activation, the oocyte were cultured in PZM-3 with 2 mmol/L 6-DMAP for 4 to 6 h and then cultured in PZM-3 without 6-DMAP for the last time. The results showed that there were no interaction between electrical field strength and pulse width(P＞0.05). When the pulse time is fixed, the cleavage rate at various electric field conditions were not significantly different(P＞0.05).When considering the electric field strength of 2.1 kV/mm and 2.5 kV/mm cleavage rate of pulse time 30 μs was significantly higher than 60 μs and 90 μs, but at the electric field strength of 2.3 kV/mm, there was no significant of cleavage rate (P＞0.05).

Key words: parthenogenesis; hypertonicity; 6-DMAP; electrical activation