›› 2012, Vol. 39 ›› Issue (1): 11-13.

• 生物技术 • Previous Articles     Next Articles

Optimization of Electroporation Processing of Porcine Rotavirus Group A Vp4 Genetic Engineering Lactobacillus

YE Li-ping, WANG Chun-feng   

  1. College of Animal Science and Technology of Jilin Agricultural University,Changchun 130118,China
  • Received:2011-06-17 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20

Abstract: In order to obtain higher electrotransformation efficiency of pW425et-Vp4 engineering Lactobacillus, studied and researched the major factors which effected the electrotransformation efficiency. The results showed the optimal values for the D600 nmof competent cell, concentration of glycine and of sucrose, the electrical field strength, the pulse time and the length of revival were 0.4, 1% (W/V), 0.3 mol/L, 11 kV/cm, 3.8 ms and 3 to 4 h, respectively, best electroporation efficiency was3.8×104 CFU/μg DNA.

Key words: porcine rotavirus group A Vp4 gene; Lactobacillus; electrotransformation

CLC Number: