沼泽型水牛卵泡抑素基因的真核表达研究

Abstract

Abstract: In this study,follistatin gene was chosen as the candidate gene affecting the reproductive ability in buffalo. Recombinant plasmid pEGFP-bFS was conducted and expressed in both buffalo fetal fibroblast (BFF) and BHK cells. pMD-bFS and pEGFP-N₁ were digested with XhoⅠ,Sac Ⅱ,then eukaryotic expression vector-pEGFP-bFS was constructed. Buffalo fetal fibroblast and BHK cells were cultured with pEGFP-bFS intermixing Lipofectamine^TM 2000 in carbon dioxide incubator with 37 ℃,5% CO₂,100% humidity. After 24 h,expression levels of recombinant vector were verified through observing directly under phase contrast fluorescence microscope,RT-PCR and Western blotting. The results indicated that bFST expressed in both buffalo fibroblast and BHK cells. This study was a very significance to produce bioactive bFS engineered plasmid and research effects of bFS on reproductive ability.

Key words: swamp buffalo; follistatin; eukaryotic expression; reproductive ability

CLC Number:

S823.8⁺3

DENG Ji-xian;YANG Xiu-rong;WEI Ying-ming;JIANG He-sheng;. Eukaryotic Expression of Swamp Buffalo Follistatin[J]. , 2011, 38(9): 57-60.

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Eukaryotic Expression of Swamp Buffalo Follistatin

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