日本乙型脑炎病毒SA14-14-2株NS1-Flag蛋白在293T细胞中的表达

Abstract

Abstract: RT-PCR was performed on the total RNA extracted from Japanese encephalitis virus (JEV)SA14-14-2 to obtain the cDNA of NS1,the cDNA was edonuclease digestion by Hind Ⅲ/HpaⅠand cloned into a vector pcDNA3.1-Flag. The recombinant plasmid was named plasmid pcDNA3.1-NS1-Flag, identified correctly by DNA sequencing and restriction enzyme analysis.The plasmid pcDNA3.1-NS1-Flag was transfected into 293T cell by means of liposome and its expression was examined by immunofluorescence assay and Western blotting,the results showed that NS1 protein could express in 293T cell.

Key words: Japanese encephalitis virus; NS1-Flag protein; eukaryotic expression; 293T cell

CLC Number:

ZANG Fu-yu;QI Wen-bao;CHEN Xiao-ming;LIU Quo-qian;LI Hong-mei;LIAO Ming. Eukaryotic Expression of NS1-Flag Protein of Japanese Encephalitis Virus SA14-14-2 in 293T Cells[J]. , 2011, 38(9): 40-43.

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Eukaryotic Expression of NS1-Flag Protein of Japanese Encephalitis Virus SA14-14-2 in 293T Cells

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