›› 2011, Vol. 38 ›› Issue (9): 125-129.

• 遗传繁育 • Previous Articles     Next Articles

Genes Modify and Expression of Porcine Interferon-beta in Yeast Pichia pasteris

TANG Ming-sen1, CHEN Rui-ai3, LIU Jian2, PEI Dang-shuai1, ZHENG Nian-guang1, LUO Man-lin1,3   

  1. 1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510462, China;2. Qilu Animal Health Products Co., Ltd., Jinan 250100, China;3. Guangdong Dahuanong Animal Health Products Co., Ltd., Xinxing 527400, China
  • Received:2011-11-22 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

Abstract: The mature nucleotide peptide of porcine interferon-β was redesigned and synthesized by high expression codon in the test,designed specific primers,porcine interferon-β was expressed through Pichia pastoris expression system,recombinant PoIFN-β was measured by cytopathic effects with Vero - VSV system, the clinical application and biological function of recombinant PoIFN-β were provided by the research.

Key words: porcine interferon-β; expression in Pichia pastoris; high activity

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