›› 2011, Vol. 38 ›› Issue (9): 115-118.

• 生物技术 • Previous Articles     Next Articles

Detection of Equine Arteritis Virus by Real-time TaqMan Reverse Transcription-PCR Assay

YANG Song1, LIANG Cheng-zhu2, GAO Hong-wei2, CHEN Pu-yan3   

  1. 1. College of Veterinary Medicine, Liaoning Medical University, Liaoning 121001, China;2. Qingdao Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China;3. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  • Received:1900-01-01 Revised:2011-03-16 Online:2011-09-20 Published:2011-09-20

Abstract: A one-step fluorogenic RT-PCR(5'-nuelease probe-based) assay using a primer/TaqMan probe set designed against a highly conserved region of open reading frame 7 (ORF7) of EAV genome was developed for the specific detection of equine arteritis virus. This assay is sensitive, fast and exact. Customs and departments of quarantine can fast and exactly examine equine arteritis virus by this assay.

Key words: quantitative detection; real-time TaqMan RT-PCR; equine arteritis viral

CLC Number: