Abstract: Two experiments were conducted to evaluate the dimethylacetylamide(DMF) and trehalose in boar semen cryopreservation protocols. In experiment 1,DMF was added at 5% to the diluents instead of glycerol used to freeze boar semen completely,evaluated lactose and trehalose on the effect of sperm cryopreservation.The results showed that compared with the lactose,trehalose increased significantly add frozen-thawed sperm viability(49.32±1.52)% and acrosome integrity(47.33%±1.16%)(P<0.05). In exprement 2,with trehalose replaced lactose,evaluated different concentrations of DMA on the cryopreservation of boar semen. The results showed that when the DMA was added at 4% level,sperm motility,viability and acrosome integrity after thawing were (45.17±0.56)%,(50.33±0.67)%,(48.30±1.44)% respectively,and were superior to 3% DMA,6% DMA(P<0.05),sperm motility was significantly higher than 5% DMA added group (P<0.05),but the sperm vitality,acrosome integrity were not significant difference (P>0.05). In conclusion,these results showed that when the use of trehalose as the basis of dilution of cryopreservation,the volume fraction of 4% DMA showed a better effect on the quality of boar cryopreservation semen.

Key words: trehalose; dimethylacetamide; boar spermatozoa; semen cryopreservation