Abstract: The aim of this paper is to improve the clinical curative effect of Chinese herbal compound(Bai hua she she cao,Honeysuckle,Astragalus,Licorice) and promote research of antiviral mechanism of action and provide the scientific basis for chinese herbal compound formulations reform and industrialized production. This study adopted orthogonal design with single factor investigation to optimize compound water extraction and alcohol precipitation technology which were follows: the weight of water extracting was referred as index for checking and choosing L₉(3⁴) orthogonal table design test to optimize the time of soaking, water consumption, decoction time, decocting number. The HPLC was used to measure content of glycyrrhizic acid of of chinese compound condensate which was referred as index for optimizing.The L₉(3⁴) orthogonal table was selected to optimize ethanol concentration, ethanol dosage and the time of alcohol precipitation. The best technology of water extraction was soaking for 1 hour,adding 12 times alcohol into Chinese medical materials, decocting 4 times and 1.5 hour each. The best technology of the alcohol precipitation was to adding 4 times 80% alcohol into extractum and then sedimenting 16 hours. With the practical production and cost factors,the technology of water extracting and alcohol sedimentating of chinese herbal compound water extracting and alcohol sedimentating was made by the way of soaking one hour,adding 12 times alcohol into chinese medical materials, decocting 3 times and 1 hour each time,adding 3 times 65% alcohol into extractum and then alcohol sedimenting 16 hours.The yield of water extraction extractum was 57.2% and the content of glycyrrhizic acid of extractum was 0.42% which all close to the maximum result in the results of orthogonal design.

Key words: Chinese herbal compound; orthegonal design; water extraction technology; alcohol sedimentation technology