Abstract: Hainan Yellow cattle peripheral blood leukocyte cDNA library was used to screen Cdc42 cDNA and pET28a-Cdc42 recombinant plasmid was constructed. Protein expression was induced by IPTG, and analyzed by SDS-PAGE and Western blotting. The results showed that the Cdc42 was composed of 576 bp, encoding 191 amino acids; the protein about 30 ku as expected was expressed after induction with IPTG; after purification,the corresponding protein strip was detected by Western blotting. The data indicated that prokaryotic expression vector of Cdc42 was constructed and expressed successfully.

Key words: Hainan Yellow cattle; Cdc42; prokaryotic expression; identification