狂犬病病毒8202株糖蛋白膜外区真核表达载体的构建及其在DK细胞中的表达

Abstract

Abstract: To construct the eukaryotic expression plasmid of glycoprotein ectodomain of rabies virus, the antigen cDNA fragment (1375 bp) of glycoprotein ectodomain of rabies virus was obtained by RT-PCR method, then it was cloned into pMD18-T vector and sequenced. Subcloned the foreign gene into the multi clone sites of pVAX1 plasmid, and identify the positive recombinat plasmid by restriction enzymes, generating pVAX-rgp. Transfected the DK cells with pVAX-rgp through Lipfectamine, identified the glycoprotein expression by Western blotting and indirect immunofluorescene assay. The results showed that the glycoprotein ectodomain could be expressed in eukaryotic cells in expressing vector. The eukaryotic expression vector for glycoprotein ectodomain gene of rabies virus was successfully constructed, which laid a foundation of study on functional genome and development on nucleic acid vaccine.

Key words: glycoprotein ectodomain of rabies virus; eukaryotic expression; transfection; DK cells

CLC Number:

YUAN Zi-guo;WANG Xiao-hu;XU Hui-juan;ZHANG Shou-feng;HU Rong-liang;ZHANG Xiu-xiang. Construction of Eukaryotic Expression Plasmid for Glycoprotein Ectodomain of Rabies Virus 8202 Strain and its Expression in DK Cells[J]. , 2011, 38(6): 60-63.

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Construction of Eukaryotic Expression Plasmid for Glycoprotein Ectodomain of Rabies Virus 8202 Strain and its Expression in DK Cells

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