Abstract: To determine the effects of Chinese traditional medicine Semen vaccariae on the proliferation and lactation fuction of bovine mammary gland epithelial cell,the water decoction was obtained from Semen vaccariae.The content of Semen vaccariae active material,dibutyl phthalate (DBP) was detected,and different concentration gradients of the drug extraction were observed for their influence on cell lactation. The most effective drug concentration was screened out for lactogenesis.The results showed that 2 mg/mL(crude drug concentration) proup was the most effective concentration for lactogenesis of bovine mammary gland epithelial cell. The results suggest that Semen vaccariae can act directly on bovine mammary gland epithelial cell for lactogenesis.

Key words: Semen vaccariae; mammary gland epithelial cell; lactation