China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (4): 30-32.

• 动物营养与饲料科学 • Previous Articles     Next Articles

Optimization of Microelement Additive Amount in Substrate for Biological Protein Feed from Cottonseed Meal during Solid State Fermentation

FENG Li1, ZHANG Wen-ju1, YU Lei1, SHI Guo-qing1,2   

  1. 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;2. Key Laboratory of Sheep Breed and Biotechnology, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi 832000, China
  • Received:2010-08-30 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

Abstract: The purpose of this study was to optimize the microelement additive amount in substrate for biological protein feed from cottonseed meal during solid state fermentation. The uniform design of 5 factors and 6 levels was applied, by measuring the free gossypol and yeast content of fermented product, to optimize the microelement additive amount in medium during solid state fermentation with Candida tropicalis ZD-3 and Saccharomyces cerevisiae ZD-5. The experimental data was analyzed to obtain the regression model and the optimal supplementary material composition was achieved by optimization with DPS v7.05 software. The optimal microelement additive amount consisted of 540 mg/kg FeSO4, 4 mg/kg CuSO4, 180 mg/kg MnSO4,15 mg/kg ZnSO4, 0 mg/kg Na2SeO3.

Key words: cottonseed meal; Candida tropicalis; Saccharomyces cerevisiae; mixed culture fermentation; biological protein feed; microelement additive amount in substrate

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