›› 2011, Vol. 38 ›› Issue (3): 73-76.

• 生物技术 • Previous Articles     Next Articles

Preparation and the Specificity of Monoclonal Antibody against Saxitoxin

YAN Dong-ming1,YU Shi-yu2,REN Hong-lin1,ZHOU Yu1,MENG Xian-mei1,LIN Chao1,LIU Zeng-shan1,LU Shi-ying1   

  1. (1.Key Laboratory of Zoonosis Research,Ministry of Education,Institute of Zoonosis,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Fuqing Entry-Exit Inspection and Quarantine Bureau,Fuqing 350300，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20
  • Contact: LU Shi-ying

Abstract: Saxitoxin（STX）was conjugated to bovine serum albumin (BSA) as immune antigen. Inoculated mouse with STX-BSA, gained one hybridoma cell strains secreting monoclonal antibodies against STX stably. The subclass of the McAb was IgM. The titer of the McAb was 1∶102400 in ascites according to the indirect ELISA determination. The affinity constant was 3.71×106 L/mol.

Key words: Saxitoxin; antigen conjugate; monoclonal antibody（McAb）; affinity constant

CLC Number: