›› 2011, Vol. 38 ›› Issue (2): 243-246.

• 经验交流 • Previous Articles     Next Articles

Study on the Culture of Balantidium coli in RPMI 1640 Medium in vitro

WANG Tian-qi1，YAN Wen-chao1，DING Ke1，ZHANG Ling1，HAN Li-fang1，WANG Xin-cai2   

  1. (1.College of Animal Livestock Science and Technology，Henan University of Science and Technology，Luoyang 471003，China;2.Medical College，Henan University of Science and Technology，Luoyang 471003，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20

Abstract: Development of the vitro culture of Balantidium coli in RPMI 1640 medium is crucial to the further study of the life cycle and pathogenetic mechanism of these intestinal protozoa. The population growth of B.coli under different culture conditions，including concentrations of starch and serum， the initial pH value and various dosages of trophozoites in RPMI 1640 medium were compared. Results indicated that 30 mg/bottle of starch and 20% serum in the RPMI 1640 medium were appropriate for culturing B.coli. The optimal initial pH value was 7.0. The natural population growth rate and the maximum population density clearly differed with different seeded doses of B.coli. These findings suggested that RPMI 1640 medium could be used to the culture of B.coli.

Key words: Balantidium coli; RPMI 1640 medium; natural population growth rate; culture in vitro

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