China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (12): 50-51.

• 生理生化 • Previous Articles     Next Articles

Study on Mutagenicity of C6 Cells HGPRT- Lines

WANG Xin1,2, SUN Shu-fang3, TAN Jian-hua4, LAI Xiao-ping2   

  1. 1. College of Animal Science,Qingdao Agricultural University,Qingdao 266109,China;2. Traditional Chinese Medical Academy of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;3. Qingdao Blood Center, Qingdal 266071,China;4. Institute of Military Veterinary Academy of Military Medical Science,PLA,Changchun 130062,China
  • Received:2011-05-09 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract: This study tries to product rat gloma cells hypox anthine-guaninep hosphoribosyl transferase deficiency lines by combined effects of N–methyl-N＇–nitro-nitrosoguanidine,8-azaguanine,6-thioguanine and ultraviolet irradiation in order to establish rat pituitary gonadotropic hormone hybridoma cell lines in future,but wo are not able to gain the expectant glioma HGPRT- cell lines which can be sieved by hypoxan thine-aminop terinthymidine selective medium.The experiment indicate that the HGPRT gene of C6 cells is too stabile to be induced deficient on this experiment conditions.

Key words: MNNG; 8-AG; 6-TG; HGPRT; C6 cells

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