蓝狐多巴胺受体D2基因部分序列的克隆测序

›› 2011, Vol. 38 ›› Issue (10): 73-75.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequencing of DRD2 Gene in Blue Fox

REN Er-jun^1,2, LIU Zong-yue¹, ZHAO Zi-yu¹, XING Xiu-mei¹, YANG Fu-he¹

1. Institute of Special Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences, Jinlin Provincial Key Laboratory for Molecular Biology of Special Economic Animals,State Key Laboratory for Molecular Biology of Special Economic Animal,Jilin 132109, China;2. Shijiazhuang Academy of Agriculture and Forestry Sciences,Shijiazhuang 050041, China

Received:2011-04-01 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

Abstract

Abstract: Dopamine receptor D2 (DRD2) gene in blue fox was cloned and sequenced,in order to provide theoretical foundation for further research of the correlation of DRD2 gene in self-biting behavior of blue fox and location. DRD2 gene of fox was amplified using particular primers,cloned by polymerase chain reaction (PCR) method and sequenced. The length of DRD2 gene in fox was 819 bp. Comparison of the fragment sequence with that of other mammal animals DRD2 gene was carried out through BLAST in GenBank. The results showed that the homologies of the nucleotide sequence with dog,mink,human and rat were 85% to 91%,DRD2 gene of blue fox was successfully cloned in this study,indicating it was quite conservative among species.

Key words: blue fox; DRD2 gene; cloning and sequencing; sequence analysis

CLC Number:

Q785

REN Er-jun;LIU Zong-yue;ZHAO Zi-yu;XING Xiu-mei;YANG Fu-he. Cloning and Sequencing of DRD2 Gene in Blue Fox[J]. , 2011, 38(10): 73-75.

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Cloning and Sequencing of DRD2 Gene in Blue Fox

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