›› 2010, Vol. 37 ›› Issue (6): 84-87.

• 生物技术 • Previous Articles     Next Articles

Establishment of the Method for Detecting Lanzhou Fat-tailed Sheep Heart Fatty Acid-binding Proteins (H-FABP) Gene mRNA with Fluorescence Quantitative Ploymerase Chain Reaction

XU Hong-wei1, ZANG Rong-xin1, YANG Ju-tian1, LU Jian-xiong1, CAI Yong2, SHI Lai-wen1, MA Wei-long1   

  1. (1.College of Life Science and Engineering of Northwest University for Nationalities,Lanzhou 730030,China;2.Science Experimental Center of Northwest University for Nationalities,Lanzhou 730030,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-20 Published:2010-06-20
  • Contact: ZANG Rong-xin

Abstract: The method for detecting Lanzhou fat-tailed sheep fatty acid-binding proteins gene mRNA expression with real time PCR was developed. By isolated total RNA form the fresh omentum, kidney weeks adipose tissue, muscle and fat tissue sample using improved the extraction Trizol one step method and reverse transcribed to cDNA using random primers as primer, the results show, the standard curve were CtH-FABP =-3.24375x+38.34230 and Ct18S=-3.33137x+33.4573, had good linear dependence, and it was sensitive and specific. The coefficient of variation values for both intra-experimental and inter-experimental reproducibility ranged less than 1.8% and 10% respectively. The method for detecting Lanzhou fat-tailed sheep H-FABP gene expression with fluorescence quantitative polymerase chain reaction was developed successfully.

Key words: Lanzhou fat-tailed sheep; fatty acid-binding proteins; SYBR Green I method; real time PCR

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