Abstract: For developing the typical testing standard of transgenic mammals and its derived food and the technical support for entry﹠exit animal quarantine, detection of the transgenic component in transgenic cow by multiplex PCR was developed in this study.The endogenous mitochondrial DNA(mtDNA) gene specific for cow identification, exotic human lysozyme(hLY)，marker NPTⅡ were amplified according to the differential primers. The process of the multiplex PCR reaction was optimized. The system described herein represent simple, accurate and sensitive detection methods in which only one reaction was necessary to detect multiple target sequences that could be reliably used for the identification of specific lines of transgenic cows.

Key words: transgene; multiplex PCR; human lysozyme