China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (6): 40-42.

• 生物技术 • Previous Articles     Next Articles

Development of Multiplex PCR Detection of Human Lysozyme Gene in Transgenic Cow

ZHU Zhen-ying1, LIN Xiang-mei1, LIU Jian1, WU Shao-qiang1, QIU Song-yin1,WANG Jian-wu2, XUE Zhen-hua3   

  1. (1. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China;2. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China; 3. Beijing Municipal Animal Husbandry and Veterinary General Station, Beijing 100107, China)
  • Received:1900-01-01 Revised:2010-04-30 Online:2010-06-20 Published:2010-06-20
  • Contact: LIU Jian

Abstract: For developing the typical testing standard of transgenic mammals and its derived food and the technical support for entry﹠exit animal quarantine, detection of the transgenic component in transgenic cow by multiplex PCR was developed in this study.The endogenous mitochondrial DNA(mtDNA) gene specific for cow identification, exotic human lysozyme(hLY)，marker NPTⅡ were amplified according to the differential primers. The process of the multiplex PCR reaction was optimized. The system described herein represent simple, accurate and sensitive detection methods in which only one reaction was necessary to detect multiple target sequences that could be reliably used for the identification of specific lines of transgenic cows.

Key words: transgene; multiplex PCR; human lysozyme

CLC Number: