Abstract: This experiment is to study the synthesis of immunogen of Sulfamethazine.The diazotization was used to link the carrier proteins BSA and OVA to SM2,and the immunogen SM2-BSA and a test coating antigen SM2-OVA were synthesized.Ultraviolet scanning and SDS-PAGE test demonstrated that the products of SM2-BSA was different from BSA and SM2,the conjugation was succeed and the conjugated ratio of SM2-BSA was 13∶1.The polyclonal antibodies with high titre and specificity were prepared by immunizing the BALB/c mice with SM2-BSA.The results indicated that the hapten of SM2 was successfully linked to carrier proteins and the polyclonal antibodies with high titre and specificity were produced.

Key words: sulfamethazine; immunogen; synthesis